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CounterCurrent Chromatography (CCC)

Countercurrent chromatography (CCC) is a liquid-liquid separation technique. Proper pairing of two immiscible liquids, one as stationary phase (SP) and the other as mobile phase (MP), effects a separation of the components of a sample through a partitioning mechanism. The SP is first pumped into the column, typically a length of plastic tubing mounted on a rotor which is spun rapidly. This high speed rotation results in a centrifugal force which is a key parameter for retention of the liquid SP. The MP is then pumped through the column, partially displacing the SP, until equilibrium is reached between the two phases.

 

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